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Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector.

Virol. J.. 2018-01; 
XuHai，BaoXi，WangYiwei，XuYue，DengBihua，LuYu，Hou
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Proteins, Expression, Isolation and Analysis … The endotoxin residue was assayed by ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (Genscript, China), data not shown. Purified phage particles were examined under an electron microscope (ZEISS, Germany) by negative staining, using 1% uranyl acetate [31] … Get A Quote

摘要

DNA delivery with bacteriophage by surface-displayed mammalian cell penetrating peptides has been reported. Although， various phages have been used to facilitate DNA transfer by surface displaying the protein transduction domain of human immunodeficiency virus type 1 Tat protein (Tat peptide)， no similar study has been conducted using T7 phage.

关键词

Bacteriophage T7，Eukaryotic expression，Targeting delivery，Vac
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