至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Coupling Green Fluorescent Protein Expression with Chemical Modification to Probe Functionally Relevant Riboswitch Conformations in Live Bacteria.

Biochemistry. 2018-08; 
DuttaDebapratim,BelashovIvan A,WedekindJose
Products/Services Used Details Operation
PCR and Cloning … of the Lrh preQ1-II riboswitch (GenScript Inc.) was sub-cloned into the pRAV23 vector (AddGene) using EcoRI and KpnI NEB restriction sites … 3a,b) was prepared in pUC57 (GenScript Inc) and sub-cloned into the pEnv8(GAAA) vector28 (AddGene) using NsiI and HindIII … Get A Quote

摘要

Noncoding RNAs engage in numerous biological activities including gene regulation. To fully understand RNA function it is necessary to probe biologically relevant conformations in living cells. To address this challenge, we coupled RNA-mediated regulation of the green fluorescent protein (GFP)uv-reporter gene to icSHAPE (in cell Selective 2'-Hydroxyl Acylation analyzed by Primer Extension). Our transcript-specific approach provides sensitive, fluorescence-based readout of the regulatory-RNA status as a means to coordinate chemical modification experiments. We chose a plasmid-based reporter compatible with Escherichia coli to allow use of knockout strains that eliminate endogenous effector biosynthesis. The ... More

关键词