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Production of biologically active feline interferon beta in insect larvae using a recombinant baculovirus.

3 Biotech. 2018-08; 
ArreguiMariana Bernadett,Mc CallumGregorio Juan,SmithIgnacio,VillaverdeMarcela Solange,WolmanFederico Javier,TargovnikAlexandra Marisa,MirandaMaría Vict
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ORF cDNA Clones/MolecularCloud … accession no. AB021707.1). The cDNA containing two restriction site sequences for EcoRI and BamHI was synthesized by GenScript (Piscataway, NJ, USA), and provided cloned in the pUC18 plasmid (pUC18-FeIFNβ). First, pUC18 … Get A Quote
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摘要

Feline interferon beta is a cytokine that belongs to the type I IFN family, with antitumor, antiviral and immunomodulatory functions. In this work, recombinant feline interferon beta (rFeIFNβ) was expressed in insect larvae that constitute important agronomic plagues. rFeIFNβ accumulated in the hemolymph of larvae infected with recombinant baculovirus and was purified by Blue-Sepharose chromatography directly from larval homogenates on day 4 post-infection. rFeIFNβ was recovered after purification with a specific activity of 1 × 10 IU mg. By this method, we obtained 8.9 × 10 IU of purified rFeIFNβ per larva. The product was biologically active in vitro, with an antiviral activity of 9... More

关键词

Baculovirus,Feline interferon beta,Insect larvae,Veterinary medi