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Membrane-permeable Rab27A is a regulator of the acrosome reaction: Role of geranylgeranylation and guanine nucleotides.

Cell. Signal.. 2018-04; 
BustosMatías A,LucchesiOrnella,RueteMaría C,TomesClaud
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Bacterial Expression System … SDS-PAGE) gels. 2.3. Construction and expression of the TAT-Rab27A. The cDNA encoding a permeable version of human Rab27A optimized for bacterial expression was synthesized by Genscript (Piscataway, NJ). The insert … Get A Quote

摘要

The acrosome reaction is the regulated exocytosis of mammalian sperm's single secretory granule, essential for fertilization. It relies on small GTPases, the cAMP binding protein Epac, and the SNARE complex, among other components. Here, we describe a novel tool to investigate Rab27-related signaling pathways: a hybrid recombinant protein consisting of human Rab27A fused to TAT, a cell penetrating peptide. With this tool, we aimed to unravel the connection between Rab3, Rab27 and Rap1 in sperm exocytosis and to deepen our understanding about how isoprenylation and guanine nucleotides influence the behaviour of Rab27 in exocytosis. Our results show that TAT-Rab27A-GTP-γ-S permeated into live spe... More

关键词

Acrosome reaction,Permeable protein,Rab27A,Rab3,