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Cross talk between β subunits, intracellular Ca signaling, and SNAREs in the modulation of Ca 2.1 channel steady-state inactivation.

Physiol Rep. 2018-01; 
SerraSelma Angèlica,GenéGemma G,Elorza-VidalXabier,Fernández-FernándezJos
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ORF cDNA Clones/MolecularCloud … Ca V 2.1 α 1A mutant subunits (ΔLI-II 451–457 , IM/EE 1964;1965 , ΔCBD 2020-end ) were generated using site-directed mutagenesis (GenScript Corporation, Piscatway, NJ). All cDNA clones used in this study were sequenced in full to confirm their integrity … Get A Quote

摘要

Modulation of Ca 2.1 channel activity plays a key role in interneuronal communication and synaptic plasticity. SNAREs interact with a specific synprint site at the second intracellular loop (LII-III) of the Ca 2.1 pore-forming α subunit to optimize neurotransmitter release from presynaptic terminals by allowing secretory vesicles docking near the Ca entry pathway, and by modulating the voltage dependence of channel steady-state inactivation. Ca influx through Ca 2.1 also promotes channel inactivation. This process seems to involve Ca -calmodulin interaction with two adjacent sites in the α carboxyl tail (C-tail) (the IQ-like motif and the Calmodulin-Binding Domain (CBD) site), and contributes to long-t... More

关键词

Ca2+-calmodulin,CaV2.1 domains for SNARE-mediated modulation,CaV2.1 steady-state inactivation,CaVβ subunits,presynaptic voltage-gated CaV2.1 channels,syntaxi