至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Divalent Cations Alter the Rate-Limiting Step of PrimPol-Catalyzed DNA Elongation.

J Mol Biol.. 2019-01; 
Xu W, Zhao W, Morehouse N, Tree MO, Zhao L.
Products/Services Used Details Operation
PCR and Cloning ...The full-length cDNA of human PrimPol (GenBank accession number BC064600) was cloned into the pET-28a(+) vector (by GenScript) between the NdeI and BamHI restriction sites as described previously... Get A Quote

摘要

PrimPol is the most recently discovered human DNA polymerase/primase and plays an emerging role in nuclear and mitochondrial genomic maintenance. As a member of archaeo-eukaryotic primase superfamily enzymes, PrimPol possesses DNA polymerase and primase activities that are important for replication fork progression in vitro and in cellulo. The enzymatic activities of PrimPol are critically dependent on the nucleotidyl-transfer reaction to incorporate deoxyribonucleotides successively; however, our knowledge concerning the kinetic mechanism of the reaction remains incomplete. Using enzyme kinetic analyses and computer simulations, we dissected the mechanism by which PrimPol transfers a nucleotide to a primer-tem... More

关键词

DNA polymerase; DNA replication; enzyme kinetics; enzyme mechanism; enzymology