Products/Services Used | Details | Operation |
---|---|---|
Monoclonal Antibody Services> | After washing, the interaction between 6His-PA(239716) and GST-PB1(125) was detected with a horseradish peroxidase-coupled anti-GST monoclonal antibody (GenScript) diluted 1:4000 in PBS supplemented with 2% FBS. | Get A Quote |
With the aim to identify small molecules able to disrupt PA-PB1 subunits interaction of influenza virus (flu) RNA-dependent RNA polymerase, and based on previous structural and computational information, in this paper we have designed and synthesized a new series of cycloheptathiophene-3-carboxamide (cHTC) derivatives. Their biological evaluation led to highlight important structural insights along with new interesting compounds, such as the 2-hydroxybenzamido derivatives 29, 31, and 32, and the 4- aminophenyl derivative 54, which inhibited viral growth in the low micromolar range (EC50 ¼ 0.18 e1.2 mM) at no toxic concentrations (CC50 > 250 mM). This study permitted to obtain among the most potent anti-... More