Products/Services Used | Details | Operation |
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PCR and Cloning> | 5-GCTCAAGAAACTCAAGAAGTTTCTGAAACAACTGAC-3 and 5-GTCAATGCTTTTTACTGCCAACAAACTCAGAC-3 for rpoC), 5 U Taq DNA polymerase (Genscript) | Get A Quote |
Drug-resistant bacterial pathogens pose an urgent public-health crisis. Here, we report the discovery, from microbial-extract screening, of a nucleoside-analog inhibitor that inhibits bacterial RNA polymerase (RNAP) and exhibits antibacterial activity against drug-resistant bacterial pathogens: pseudouridimycin (PUM). PUM is a natural product comprising a formamidinylated, N-hydroxylated Gly-Gln dipeptide conjugated to 6'-amino-pseudouridine. PUM potently and selectively inhibits bacterial RNAP in vitro, inhibits bacterial growth in culture, and clears infection in a mouse model of Streptococcus pyogenes peritonitis. PUM inhibits RNAP through a binding site on RNAP (the NTP addition site) and mechanism (competi... More