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Monomeric Camelus dromedarius GSTM1 at low pH is structurally more thermostable than its native dimeric form.

PLoS One.. 2018-10; 
Malik A, Khan JM, Alamery SF, Fouad D, Labrou NE, Daoud MS, Abdelkader MO, Ataya FS.
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Gene Synthesis number HM475154), cloned in pET-3a(+) expression vector under the control of T7 promotor (Genscript) ...Recombinant CdGSTM1 was purified using GSH-agarose adsorbent (2 mL; Genscript). Get A Quote
Nucleic Acid Purification & Analysis Get A Quote

摘要

Glutathione S‒transferases (GSTs) are multifunctional enzymes that play an important role in detoxification, cellular signalling, and the stress response. Camelus dromedarius is well-adapted to survive in extreme desert climate and it has GSTs, for which limited information is available. This study investigated the structure-function and thermodynamic properties of a mu-class camel GST (CdGSTM1) at different pH. Recombinant CdGSTM1 (25.7 kDa) was expressed in E. coli and purified to homogeneity. Dimeric CdGSTM1 dissociated into stable but inactive monomeric subunits at low pH. Conformational and thermodynamic changes during the thermal unfolding pathway of dimeric and monomeric CdGSTM1 were characterised via ... More

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