In this study, we have generated a dual expression
cassette plasmid DNA (pDNA) construct containing the
species-specific horse (Equus caballus) codon optimized sequence encoding potent pro-angiogenic vascular endothelial
growth factor (VEGF164) and basic fibroblast growth factor
(FGF2) under eukaryotic promoters (EF-1α and CMV promoters, respectively). We have demonstrated effective and
simultaneous recombinant proteins expression in vitro.
Resulting pDNA is suitable for potential gene therapy applications in horses
In this study, we have generated a dual expression
cassette plasmid DNA (pDNA) construct containing the
species-specific horse (Equus caballus) codon optimized sequence encoding potent pro-angiogenic vascular endothelial
growth factor (VEGF164) and basic fibroblast growth factor
(FGF2) under eukaryotic promoters (EF-1α and CMV promoters, respectively). We have demonstrated effective and
simultaneous recombinant proteins expression in vitro.
Resulting pDNA is suitable for potential gene therapy applications in horses