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Generation of Plasmid DNA Expressing Species-Specific Horse VEGF164 and FGF2 Factors for Gene Therapy

BioNanoSci.. 2016-01;

Y. A. Litvin & E. Yu Zakirova & M. N. Zhuravleva1 & A. A. Rizvanov

Products/Services Used	Details	Operation
Molecular Biology Tools	A starting coding sequences of horse vegf164 (NCBI Reference Sequence: NM_001081821.1) and fgf2 (NCBI Reference Sequence: NM_001195221.1) were codon optimized using algorithm BOptimumGene^ (GenScript, USA),	Get A Quote

摘要

In this study, we have generated a dual expression cassette plasmid DNA (pDNA) construct containing the species-specific horse (Equus caballus) codon optimized sequence encoding potent pro-angiogenic vascular endothelial growth factor (VEGF164) and basic fibroblast growth factor (FGF2) under eukaryotic promoters (EF-1α and CMV promoters, respectively). We have demonstrated effective and simultaneous recombinant proteins expression in vitro. Resulting pDNA is suitable for potential gene therapy applications in horses