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Mesothelin promoter variants are associated with increased soluble mesothelin-related peptide levels in asbestos-exposed individuals

Occup Environ Med. 2017-06;

De SantiChiara, PucciPerla, BonottiAlessandra, MelaiuOmbretta, CipolliniMonica, SilvestriRoberto, VymetalkovaVeronika, BaroneElisa, PaolicchiElisa, CorradoAlda, LeporiIrene, Dell'AnnoIrene, PellèLucia, VodickaPavel, MuttiLuciano, FoddisRudy, CristaudoAlfonso, GemignaniFederica, LandiSte

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PCR and Cloning	… was selected from our sample set. The resultant polymerase chain reaction (PCR) amplicon was subsequently cloned into the XhoI site of the pGL3-basic vector (Promega, Madison, WI) using CloneEZ® PCR Cloning Kit (GenScript, Piscataway, USA). This construct, Page 7 …	Get A Quote

摘要

Soluble mesothelin-related peptide (SMRP) is a promising diagnostic biomarker for malignant pleural mesothelioma (MPM)， but various confounders hinder its usefulness in surveillance programmes. We previously showed that a single nucleotide polymorphism (SNP) within the 3'untranslated region (3'UTR) of the () gene could affect the levels of SMRP.