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B cell and antibody responses in mice induced by a putative cell surface peptidase of Pneumocystis murina protect against experimental infection

Vaccine. 2017-01;

RuanSanbao, CaiYang, RamsayAlistair J, WelshDavid A, NorrisKaren, ShellitoJu

Products/Services Used	Details	Operation
Peptide Synthesis	… 2.5. ELISA reactivity of mouse and monkey serum to SPD1 peptides. Overlapping peptides covering the SPD1 sequence were synthesized by GenScript (Piscataway, NJ) … N-230 & C-450 were expressed in a Baculovirus by GenScript (Piscataway, NJ) …	Get A Quote
Insect Expression System		Get A Quote

摘要

Pneumocystis pneumonia is a major cause of morbidity and mortality in HIV-infected subjects， cancer patients undergoing chemotherapy and solid organ transplant recipients. No vaccine is currently available. By chemical labeling coupled with proteomic approach， we have identified a putative surface protein (SPD1， Broad Institute gene accession number PNEG_01848) derived from single suspended P. murina cysts. SPD1 was expressed in an insect cell line and tested for vaccine development.