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Development of Real-Time and Conventional PCR Assays for Identifying Stubby Root Nematode Paratrichodorus allius

Plant Dis.. 2017-06; 
HuangDanqiong, YanGuiping, SkantarAndr
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PCR and Cloning … Tanha Maafi et al. (2003). Three plasmid DNA extracted from positive colonies for each population were submitted for sequencing. The sequencing information was provided by Genscript (Piscataway, NJ). A Blastn alignment was … Get A Quote

摘要

Paratrichodorus allius is an important pest on many crops, particularly on potato due to its ability to transmit Tobacco rattle virus causing corky ringspot disease on tubers. Detection and identification of P. allius are important for effective disease management. In this study, a rapid and reliable molecular diagnosis of this nematode targeting internal transcribed spacer ribosomal DNA was established. The specificity of the designed primers was evaluated using 29 nematode species and results showed that a single amplicon was produced from DNA of P. allius only. Detection sensitivity analysis indicated that a 9.6 × 10 ng of DNA template could be detected by conventional PCR and 1.92 × 10 ng of DNA by re... More

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