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Rapid detection and evolutionary analysis of Legionella pneumophila serogroup 1 sequence type 47

Clin. Microbiol. Infect.. 2017-04;

MentastiM, CassierP, DavidS, GinevraC, Gomez-ValeroL, UnderwoodA, AfsharB, EtienneJ, ParkhillJ, ChalkerV, BuchrieserC, HarrisonT G, Jarra

Products/Services Used	Details	Operation
PCR and Cloning	… value. Efficiency and limit of detection. A custom-made plasmid, pRSBS, containing the PCR targets was designed and purchased from GenScript (Piscataway, USA), and used to calculate efficiency and the limit of detection …	Get A Quote

摘要

Legionella pneumophila serogroup 1 (Lp1) sequence type 47 is the leading cause of legionellosis in north-western Europe， but， surprisingly， it is rarely isolated from environmental samples. Comparative genomics was applied to develop a PCR assay and to better understand the evolution of this strain.