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Nucleic Acid Purification & Analysis> | … 0.25M Tris-HCl, pH 6.8, 8% SDS, 20% b-mercaptoethanol, 40% glycerol and 0.04% bromophenol blue) followed by boiling for 10min at 95 C. Twenty microliters of each sample was loaded onto the precasted 4-15% gradient SDS-PAGE gels (GenScript, Piscataway, NJ, USA) … | Get A Quote |
Camels have exceptional carbohydrate metabolism as their plasma glucose level is high and have low whole body insulin sensitivity, similar to that observed in type 2 diabetes patients. We aimed at studing an important component of insulin signalling pathway, the GLUT4, in camel. Camelus dromedarius GLUT4 (CdGLUT4) CDS is 1530 nucleotide in length that encodes for a 55KDa protein. CdGLUT4 has 23 amino acid substitutions and 3N-glycosylation sites, compared to 2 in Human GLUT4. 3 D structures of CdGLUT4 and HsGLUT4 generated by homology modelling revealed conservation of characteristic signature motifs. CdGLUT4 was cloned and expressed optimally in C43(DE3)pLysS strain and maximum detergent solubility w... More