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Amiloride-enhanced gene transfection of octa-arginine functionalized calcium phosphate nanoparticles

PLoS ONE. 2017-01; 
Vanegas SáenzJuan Ramón, TenkumoTaichi, KamanoYuya, EgusaHiroshi, SasakiKei
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PCR and Cloning … into a small beaker using a peristaltic pump in a Ca:P ratio of 1.6:1. From this dispersion, 50 μl was collected with a pipette and rapidly mixed with 20 μl of an aqueous solution of the AcGFP1 plasmid encoding a green fluorescent protein (1 mg ml −1 ) (GenScript, USA Inc.) or a … Get A Quote

摘要

Nanoparticles represent promising gene delivery systems in biomedicine to facilitate prolonged gene expression with low toxicity compared to viral vectors. Specifically, nanoparticles of calcium phosphate (nCaP), the main inorganic component of human bone, exhibit high biocompatibility and good biodegradability and have been reported to have high affinity for protein or DNA, having thus been used as gene transfer vectors. On the other hand, Octa-arginine (R8), which has a high permeability to cell membrane, has been reported to improve intracellular delivery systems. Here, we present an optimized method for nCaP-mediated gene delivery using an octa-arginine (R8)-functionalized nCaP vector contai... More

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