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PCR and Cloning> | … and L3 coding sequences (NCBI Reference Sequences ERY59288, ERZ09841.1, WP_003115311, CDG56231.1, ERX71449.1, and ERZ04935.1, respectively) optimized for expression in the host plant Nicotiana benthamiana were synthetized by GenScript (USA) and inserted … | Get A Quote |
The emergence, persistence and spread of antibiotic-resistant human pathogenic bacteria heralds a growing global health crisis. Drug-resistant strains of gram-negative bacteria, such as Pseudomonas aeruginosa, are especially dangerous and the medical and economic burden they impose underscore the critical need for finding new antimicrobials. Recent studies have demonstrated that plant-expressed bacteriocins of the colicins family can be efficient antibacterials against all major enteropathogenic strains of E. coli. We extended our studies of colicin-like bacteriocins to pyocins, which are produced by strains of P. aeruginosa for ecological advantage against other strains of the same species. Using a pla... More