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Rotavirus genotype shifts among Swedish children and adults-Application of a real-time PCR genotyping

J. Clin. Virol.. 2017-11; 
AnderssonMaria, LindhMa
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PCR and Cloning … C for 30 min followed by 10 min of denaturation at 95 °C, 45 cycles of two-step PCR was performed (15 s at 95 °C, 60 s at 58 °C) with the ramp rate adjusted to 1 °C/s. A pUC57 plasmid containing the target regions for all real-time PCR systems (GenScript, Piscataway, NJ) was … Get A Quote

摘要

It is well known that human rotavirus group A is the most important cause of severe diarrhoea in infants and young children. Less is known about rotavirus infections in other age groups， and about how rotavirus genotypes change over time in different age groups.

关键词

Adults，Children，Gastroenteritis，Genotypes，Real-time PCR，Rotav
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