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Regulation of early growth response 2 expression by secreted frizzled related protein 1

BMC Cancer. 2017-07; 
GregoryKelly J, MorinStephanie M, SchneiderSall
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PCR and Cloning … Additionally, primer sequences were designed to cross exon junctions using GenScript Real-time PCR Primer Design (www.genscript.com/tools/real-time-pcr-tagman-primer-design- tool) and are as follows: human EGR2 forward: 5′-TCCCAGTAACTCTCAGTGGTT-3′, human … Get A Quote

摘要

Secreted frizzled-related protein 1 (SFRP1) expression is down-regulated in a multitude of cancers, including breast cancer. Loss of Sfrp1 also exacerbates weight gain as well as inflammation. Additionally, loss of SFRP1 enhances TGF-β signaling and the downstream MAPK pathway. TGF-β has been shown to increase the expression of Early Growth Response 2 (EGR2), a transcription factor implicated in immune function in a wide variety of cell types. The work described here was initiated to determine whether SFRP1 modulation affects TGF-β mediated EGR2 expression in mammary tissues as well as macrophage polarization.

关键词

EGR2,MAPK,Macrophage polarization,Mammary gland,SFRP1,TG