Products/Services Used | Details | Operation |
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PCR and Cloning> | … laboratory collection (Chikh-Ali et al. 2013, 2016c). The PCR step was carried out using GreenTaq DNA Polymerase (GenScript, Piscataway, NJ) as previously described (Chikh-Ali et al. 2013). All 12 samples were also screened … | Get A Quote |
Potato is one of the staple crops in Egypt, grown under irrigation almost continuously year-round. Potato virus Y (PVY) has been reported as one of the main viruses affecting potatoes in Egypt, but limited information is available on PVY strains circulating in potato fields in the country. From 2014 to 2016, virus surveys were conducted in several potato-growing governorates of Egypt, and PVY-positive samples were found to represent at least five distinct recombinant PVY strains, including PVY and PVY. Whole genome sequences were determined for four isolates representing strains PVY-SYR-III (Egypt7), PVY-261-4 (Egypt11), PVY (Egypt35), and a novel recombinant named Egypt24 that combined molecula... More