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Somatic cell selection for chlorsulfuron-resistant mutants in potato: identification of point mutations in the acetohydroxyacid synthase gene.

BMC Biotechnol.. 2017-06; 
BarrellPhilippa J, LatimerJulie M, BaldwinSamantha J, ThompsonMichelle L, JacobsJeanne M E, ConnerAntho
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PCR and Cloning … previously described [31]. The AHAS coding sequence flanked by the Lhca3 promoter and terminator was obtained from Genscript Corporation (Piscataway, NJ, USA) as a fragment ligated into the plasmid pUC57. The chimeric … Get A Quote

摘要

Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in plants. This study undertook somatic cell selection in the potato (Solanum tuberosum L.) cultivar 'Iwa' using the sulfonylurea herbicide， chlorsulfuron， as a positive selection agent.

关键词

Acetohydroxyacid synthase，Acetolactate synthase，Chlorsulfuron resistance，Intragenic selectable marker，Potato，Somatic cell selection，Sulfonylurea resist
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