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Spectral and thermal properties of novel eye lens ζ-crystallin.

Int. J. Biol. Macromol.. 2017-09; 
MalikAjamaluddin, AlbogamiShurog, AlsenaidyAbdulrahman M, AldbassAbeer M, AlsenaidyMohammad A, KhanShams Ta
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Nucleic Acid Purification & Analysis … All other chemicals used in this study were reagent grade. 4–20% precasted SDS–PAGE gradient gel was from Genscript. Circular dichroism spectroscopy (CD) from Applied Photophysics. Cary eclipse spectrofluorometer was from Agilent Technologies. 2.2 … Get A Quote

摘要

Eye lenses are exposed to thermal, solar radiations, dryness that enhances cataractogenesis. Some animal lenses contain novel proteins in bulk quantities. ζ-crystallin occurred in three ecologically divergent species, but it's physiological role not known. The truncated variant of ζ-crystallin causes hereditary cataract. Guinea pig ζ-crystallin is temperature-sensitive and rapidly aggregates at 41°C. Camels adopted to survive above 50°C, which raises an interesting question about how it retains lens proteins in the soluble state? Here, we have optimized expression and purification of recombinant camel ζ-crystallin. We have studied thermodynamic and spectroscopic properties using orthogonal techn... More

关键词

Cataract,Dynamic multimode spectroscopy,Thermal shift assay,ζ-Crysta