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Genetic basis for variation in salinity tolerance between stickleback ecotypes.

Mol. Ecol.. 2017-01; 
KusakabeMakoto, IshikawaAsano, RavinetMark, YoshidaKohta, MakinoTakashi, ToyodaAtsushi, FujiyamaAsao, Kitan
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PCR and Cloning … Applied Biosystems). Primer sequences in Table S1 (Supporting information) were designed using a primer design online tool (GenScript Real‐time PCR Primer Design, https://www.genscript.com/ssl-bin/app/primer). PCR was … Get A Quote

摘要

Adaptation to different salinities can drive and maintain divergence between populations of aquatic organisms. Anadromous and stream ecotypes of threespine stickleback (Gasterosteus aculeatus) are an excellent model to explore the genetic mechanisms underlying osmoregulation divergence. Using a parapatric pair of anadromous and stream stickleback ecotypes, we employed an integrated genomic approach to identify candidate genes important for adaptation to different salinity environments. Quantitative trait loci (QTL) mapping of plasma sodium concentrations under a seawater challenge experiment identified a significant QTL on chromosome 16. To identify candidate genes within this QTL, we first conducted RNA-se... More

关键词

ATPase,FST differentiation,anadromous,osmoregula