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Prevalence and Genetic Characterization of Infection in Java Sparrows () in Northern China.

Biomed Res Int. 2017-01; 
YaoQiu-Xia, ZhangXiao-Xuan, ChenKai, MaJian-Gang, ZhengWen-Bin, XuXiao-Qin, ZhuXing-
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PCR and Cloning … PCR products were observed under UV light after electrophoresis in 1.5% agarose gel containing GoldView™ (Solarbio, Beijing, China). 2.4. Sequencing Analyses. The positive PCR products of Java sparrows were sequenced by GenScript Company (Nanjing, China) … Get A Quote

摘要

Cryptosporidiosis is a cosmopolitan parasitosis that affects a wide range of hosts including birds. As information concerning in birds is limited, the present study examined the prevalence and genotypes of in Java sparrows in Beijing and Shangqiu, northern China. Three hundred and fifty fecal samples were collected from Java sparrows (, 225 white Java sparrows and 125 gray Java sparrows) in Beijing and Shangqiu in October 2015, and the samples were examined by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) gene. The overall prevalence is 13.42% (47/350), with 16.44% (37/225) in white Java sparrows and 8.00% (10/125) in gray Java sparrows. prevalence was 9.82% (16/163) in Java spar... More

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