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PCR Cloning and Subcloning> | … TOP10). Positive clones were selected and sequenced using an ABI model 3730 DNA sequencer (GenScript, Nanjing; China). Sequence similarity analysis was performed by using the Ezbioclound server (Yoon et al. 2017) … | Get A Quote |
In this study, a Gram-negative, rod-shaped, endophytic bacterial strain (RS1) capable of producing large amounts of exopolysaccharides was isolated from a stem of Miscanthus sinensis. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RS1 is closely related to Mucilaginibacter kameinonensis NBRC 102645 (98.72%), followed by Mucilaginibacter gossypiicola Gh-48 (97.56%) and Mucilaginibacter oryzae DSM 19975 (97.36%). The DNA G + C content of strain RS1 was determined to be 42.80 mol%. ANIb and GGDC values from genomic comparison between the genomes of strain RS1 and the related reference species were less than 95% and 70%, respectively. The major cellular fatty acids (more ... More