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Bm-iAANAT3: Expression and characterization of a novel arylalkylamine N-acyltransferase from Bombyx mori.

Arch. Biochem. Biophys.. 2019-01; 
BattistiniMatthew R, O'FlynnBrian G, ShojiChristopher, SuarezGabriela, GallowayLamar C, MerklerDav
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Custom DNA/RNA Oligos … Codon-optimized Bm-iAANAT3 was purchased from Genscript. Oligonucleotides were purchased from Eurofins MWG Operon … Kanamycin monosulfate and isopropyl-β-d- thiogalactopyranoside (IPTG) were purchased from Gold Biotechnology … Get A Quote

摘要

The arylalkylamine N-acyltransferases (AANATs) are enzymes that catalyze the acyl-CoA-dependent formation of N-acylarylalkylamides: acyl-CoA + arylalkylamine → N-acylarylalkylamides + CoA-SH. Herein, we describe our study of a previously uncharacterized AANAT from Bombyx mori: Bm-iAANAT3. Bm-iAANAT3 catalyzes the direct formation of N-acylarylalkylamides and accepts a broad range of short-chain acyl-CoA thioesters and amines as substrates. Acyl-CoA thioesters possessing an acyl chain length >10 carbon atoms are not substrates for Bm-iAANAT3. We report that Bm-iAANAT3 is a "versatile generalist", most likely, functioning in amine acetylation - a reaction in amine inactivation/excretion, cuticle scl... More

关键词

AANAT,Bombyx mori,Chemical mechanism,Kinetic mechanism,N-acylarylakyla