Flavonoids with various structures play a vital role in plant acclimatization to varying environments as well as in plant growth， development， and reproduction. Exogenous applications of ethylene and 1-aminocyclopropane carboxylic acid (ACC)， could affect the accumulation of flavonoids. Very few attempts have been made to investigate the effect of 1-aminocyclopropane carboxylic acid oxidase (ACO)， a unique enzyme that catalyzes ACC to ethylene， on genes and metabolites in the flavonoid biosynthetic pathway. In this study， two ACOs in safflower (CtACOs) were cloned， and then transgenic safflower with overexpressed CtACO1 was generated through the Agrobacterium-mediated floral dipping method.
Flavonoids with various structures play a vital role in plant acclimatization to varying environments as well as in plant growth， development， and reproduction. Exogenous applications of ethylene and 1-aminocyclopropane carboxylic acid (ACC)， could affect the accumulation of flavonoids. Very few attempts have been made to investigate the effect of 1-aminocyclopropane carboxylic acid oxidase (ACO)， a unique enzyme that catalyzes ACC to ethylene， on genes and metabolites in the flavonoid biosynthetic pathway. In this study， two ACOs in safflower (CtACOs) were cloned， and then transgenic safflower with overexpressed CtACO1 was generated through the Agrobacterium-mediated floral dipping method.
