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Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex.

BMC Biotechnol.. 2019-01; 
HaoZhenzhen, SuXia
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PCR and Cloning … To determine the ability to guide digestion, 1 μg of gRNA, 2 μg of recombinant Cas9 (GenScript, Nanjing, China), and 200 ng of the BcuI-linearized pT3cbh1 plasmid were mixed and incubated at 37 °C for 2 h. At the end of reaction, the samples were taken out for agarose gel … Get A Quote

摘要

CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei， a filamentous fungus workhorse for cellulase production. However， expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome.

关键词

CRISPR/Cas9，Gene disruption，Ribonucleoprotein，Trichoderma re
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