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PCR and Cloning> | … 89 enzymes (BamH I and Hind III) and then inserted into the plasmid pRNA-H1.1 90 (GenScript, Nanjing, China). The target sequence for shRNF2 was as follows: 91 5'-ACGGAACTCAACC ATTAAG-3'. The resulting recombinant plasmid was 92 named pRNA-H1.1-shRNF2 … | Get A Quote |
A previous study has reported that RING finger protein 2 (RNF2) knockdown increases radiosensitivity of esophageal cancer cells both in vitro and in vivo. However, the effect of RNF2 knockdown on radiosensitivity in squamous cell carcinoma (SqCC) remains unknown. NCI-H226 and SK-MES-1 cells were exposed to X-ray irradiation and then RNF2 levels were determined. RNF2 was knocked down and stable transfectants were selected. Radiosensitivity, cell proliferation, apoptosis, cell cycle and γ-H2AX foci formation were evaluated. Interaction among ataxia telangiectasia mutated protein (ATM), mediator of DNA damage checkpoint 1 (MDC1) and H2AX was examined. Xenograft models were used to explore the effect of ... More