Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | … 17 4 Biotechnology Development Laboratories, Health Care Solutions Research Institute, Kaneka 18 Corporation, 1-8 Takasago-shi, Hyogo 676-8688, Japan 19 20 … 1563). The oligonucleotides and their complements were synthesized by GenScript Japan Inc. 97 … | Get A Quote |
RNA interference (RNAi) can inhibit Influenza A virus (IAV) infection in a gene-specific manner. In this study, we constructed a transgene expressing a short hairpin RNA (shRNA) that targets the noncoding region of the IAV RNA gene encoding nucleoprotein (NP). To investigate the antiviral effects of the shRNA, we generated two transgenic mouse lines with this transgene. Unfortunately, there was no apparent difference in IAV resistance between transgenic and non-transgenic littermates. To further investigate the antiviral effects of the shRNA, we prepared mouse embryonic fibroblasts (MEFs) from transgenic and non-transgenic mice. In experimental infections using these MEFs, virus production of mouse-ad... More