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Tank binding kinase 1 is a centrosome-associated kinase necessary for microtubule dynamics and mitosis.

Nat Commun. 2015-12; 
Pillai S, Nguyen J, Johnson J, Haura E, Coppola D, Chellappan S.
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Mutagenesis Services C terminus of CEP170-containing 2,331 nucleotides (777 amino acids) was synthesized (Genscript) with all serine and threonine residues targeted by TBK1 mutated to alanine or valine, respectively Get A Quote

摘要

TANK Binding Kinase 1 (TBK1) is a non-canonical IκB kinase that contributes to KRAS-driven lung cancer. Here we report that TBK1 plays essential roles in mammalian cell division. Specifically, levels of active phospho-TBK1 increase during mitosis and localize to centrosomes, mitotic spindles and midbody, and selective inhibition or silencing of TBK1 triggers defects in spindle assembly and prevents mitotic progression. TBK1 binds to the centrosomal protein CEP170 and to the mitotic apparatus protein NuMA, and both CEP170 and NuMA are TBK1 substrates. Further, TBK1 is necessary for CEP170 centrosomal localization and binding to the microtubule depolymerase Kif2b, and for NuMA binding to dynein. Finally, selecti... More

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