| Products/Services Used | Details | Operation |
|---|---|---|
| PCR Cloning and Subcloning | . All genes were highly expressed with the CMVp (cytomegalovirus promoter) in mammalian cells. All plasmid manipulations were performed by Genscript.Using the base structure of our cassette plasmid,34 the transfer vectors for TF-RCaMP, TFCaRQ, Null, RCaMP, and CaRQ cells were synthesized by Genscript (Pescataway, NY) and subcloned into the pUC57- Simple vector using EcoRV. | Get A Quote |
Magnetoreception can be generally defined as the ability to transduce the effects of a magnetic field into a cellular response. Magnetic stimulation at the cellular level is particularly attractive due to its ability for deep penetration and minimal invasiveness， allowing remote regulation of engineered biological processes. Previously， a magnetic-responsive genetic circuit was engineered using the transient receptor potential vanilloid 1 (TRPV1) and the iron containing ferritin protein (i.e.， the TF circuit). In this study， we combined the TF circuit with a Ca activated RhoA protein (CaRQ) to allow a magnetic field to remotely regulate cell migration. Cells expressing the TF circuit and CaRQ exhibited ... More
