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The gating mechanism in cyclic nucleotide-gated ion channels.

Sci Rep. 2018; 
MazzoliniMonica,ArcangelettiManuel,MarchesiArin,NapolitanoLuisa M R,GrosaDebora,MaitySourav,AnselmiClaudio,TorreVin
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摘要

Cyclic nucleotide-gated (CNG) channels mediate transduction in several sensory neurons. These channels use the free energy of CNs' binding to open the pore, a process referred to as gating. CNG channels belong to the superfamily of voltage-gated channels, where the motion of the α-helix S6 controls gating in most of its members. To date, only the open, cGMP-bound, structure of a CNG channel has been determined at atomic resolution, which is inadequate to determine the molecular events underlying gating. By using electrophysiology, site-directed mutagenesis, chemical modification, and Single Molecule Force Spectroscopy, we demonstrate that opening of CNGA1 channels is initiated by the format... More

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