It is well known that iron is an essential element for all living organism. The intracellular iron availability is also important for the host's innate immune response to various pathogens， in which the iron homeostasis can be regulated by ferritin due to its iron storage property. In this study， a full-length cDNA sequence of ferritin (named as CqFerritin) was identified with 1410 bp from red claw crayfish Cherax quadricarinatus， which contained an open reading frame of 513 bp， encoding 170 amino acids with a conserved ferritin domain. Tissue distribution analysis demonstrated that CqFerritin was widely expressed in various tissues with high presence in haemocyte， haematopoietic tissue (Hpt) and hear... More
It is well known that iron is an essential element for all living organism. The intracellular iron availability is also important for the host's innate immune response to various pathogens， in which the iron homeostasis can be regulated by ferritin due to its iron storage property. In this study， a full-length cDNA sequence of ferritin (named as CqFerritin) was identified with 1410 bp from red claw crayfish Cherax quadricarinatus， which contained an open reading frame of 513 bp， encoding 170 amino acids with a conserved ferritin domain. Tissue distribution analysis demonstrated that CqFerritin was widely expressed in various tissues with high presence in haemocyte， haematopoietic tissue (Hpt) and heart， while lowest expression in hepatopancreas. In addition， loss-of-function of CqFerritin by gene silencing resulted in significantly higher expression of an envelope protein VP28 of white spot syndrome virus (WSSV) in red claw crayfish Hpt cell cultures， indicating the potential antiviral response of CqFerritin. To further explore the effect on WSSV replication by CqFerritin， recombinant CqFerritin protein (rCqFerritin) was transfected into Hpt cells followed by WSSV infection. Importantly， the replication of WSSV was obviously decreased in Hpt cells if transfected with rCqFerritin protein， suggesting that CqFerritin had clearly negative effect on WSSV infection. Furthermore， intracellular accumulation of iron ions was found to promote the WSSV replication in a dose-dependent manner， illustrating that the iron level regulated by CqFerritin was likely to be vital for WSSV infection in red claw crayfish. Taken together， these data suggest that CqFerritin plays an important role in immune defense against WSSV infection in a crustacean C. quadricarinatus.
