| Products/Services Used | Details | Operation |
|---|---|---|
| Mutagenesis Services | To generate the UAS-pHluorin-Rab2 and UAS-pHluorin-Rab2Q65L transgenes the mCherry cassette in the UAS-mCherry-Rab2 and UAS-mCherry-Rab2Q65L plasmids, respectively, was exchanged for a super-ecliptic pHluorin cassette using the GenScript gene editing service.Likewise, to generate the genomic GFP-Rab2 transgene the HA coding sequence in the pCasper4-HA-Rab2 construct was exchanged for a EGFP coding sequence using the GenScript gene editing service. All DNA work for CRISPR/Cas9 mediated mutagenesis was performed by GenScript. | Get A Quote |
| Gene Synthesis | Get A Quote | |
| PCR Cloning and Subcloning | Get A Quote |
Rab2 is a conserved Rab GTPase with a well-established role in secretory pathway function and phagocytosis. Here we demonstrate that Drosophila Rab2 is recruited to late endosomal membranes， where it controls the fusion of LAMP-containing biosynthetic carriers and lysosomes to late endosomes. In contrast， the lysosomal GTPase Gie/Arl8 is only required for late endosome-lysosome fusion， but not for the delivery of LAMP to the endocytic pathway. We also find that Rab2 is required for the fusion of autophagosomes to the endolysosomal pathway， but not for the biogenesis of lysosome-related organelles. Surprisingly， Rab2 does not rely on HOPS-mediated vesicular fusion for recruitment to late endosomal memb... More
