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A Kinesin-14 Motor Activates Neocentromeres to Promote Meiotic Drive in Maize.

Cell. 2018;

DaweR Kelly，LowryElizabeth G，GentJonathan I，StitzerMichelle C，SwentowskyKyle W，HigginsDavid M，Ross-IbarraJeffrey，WallaceJason G，KanizayLisa B，AlabadyMagdy，QiuWeihong，TsengKuo-Fu，WangNa，GaoZhi，BirchlerJames A，HarkessAlex E，HodgesAmy L，HiattEvel

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	The sequence (CCCCCCTTTCCAGTTCCATCCGAATCGAGAGGCCGAGGCGCCCGGATCCACCGCTCACCCC CGCTCCTCAAACCTCCATGGATCCACCGCTCACCTCCGCTCCTCAAACCTCCATGGATTCACCCGCCATGCCCGCGCGACGGAG GCGCCCGGAGGTTCTACACGACAAGGAGAACCCAGCGGACGCGCAGCCCGGAGAGCGCCAGCGCACCGCTGCCGGAACCGG GAGGCAGCCACTTGCGGGCGCGCTGCCGCCGCCGCCGCCGGAGTCTTTGCAAAATGAAGCTGATTTATTGAAGGAGGTTGAAA) was synthesized in both orientations by Genscript (Piscataway, NJ) with restriction site adapters added to the ends, such that the reverse sequence was cloned into the vector with AscI and AvrII, and the forward sequence was cloned with SacI and SpeI.The cDNAs of Kindr and ZmKin11 were synthesized by Genscript and are shown in Table S3.	Get A Quote

摘要

Maize abnormal chromosome 10 (Ab10) encodes a classic example of true meiotic drive that converts heterochromatic regions called knobs into motile neocentromeres that are preferentially transmitted to egg cells. Here， we identify a cluster of eight genes on Ab10， called the Kinesin driver (Kindr) complex， that are required for both neocentromere motility and preferential transmission. Two meiotic drive mutants that lack neocentromere activity proved to be kindr epimutants with increased DNA methylation across the entire gene cluster. RNAi of Kindr induced a third epimutant and corresponding loss of meiotic drive. Kinesin gliding assays and immunolocalization revealed that KINDR is a functional minus-end-d... More