Extraintestinal pathogenic Escherichia coli (ExPEC) is one of the leading causes of bloodstream infections. Characteristically， these organisms exhibit strong resistance to the bactericidal action of host serum. Although numerous serum resistance factors in ExPEC have been identified， their regulatory mechanisms during in vivo infection remain largely unknown. Here， RNA sequencing analyses together with quantitative reverse-transcription PCR revealed that ExPEC genes involved in the biosynthesis of extracytoplasmic polysaccharides (ECPs) including K-capsule， lipopolysaccharide (LPS)， colanic acid， peptidoglycan， and Yjb exopolysaccharides were significantly upregulated in response to serum under l... More
Extraintestinal pathogenic Escherichia coli (ExPEC) is one of the leading causes of bloodstream infections. Characteristically， these organisms exhibit strong resistance to the bactericidal action of host serum. Although numerous serum resistance factors in ExPEC have been identified， their regulatory mechanisms during in vivo infection remain largely unknown. Here， RNA sequencing analyses together with quantitative reverse-transcription PCR revealed that ExPEC genes involved in the biosynthesis of extracytoplasmic polysaccharides (ECPs) including K-capsule， lipopolysaccharide (LPS)， colanic acid， peptidoglycan， and Yjb exopolysaccharides were significantly upregulated in response to serum under low oxygen conditions and during bloodstream infection. The oxygen sensor FNR directly activated the expression of K-capsule and colanic acid and also indirectly modulated the expression of colanic acid， Yjb exopolysaccharides， and peptidoglycan via the known Rcs regulatory system. The global regulator Fur directly or indirectly repressed the expression of ECP biosynthesis genes in iron replete media， whereas the low iron conditions in the bloodstream could relieve Fur repression. Using in vitro and animal models， FNR， Fur， and the Rcs system were confirmed as contributing to ExPEC ECP production， serum resistance， and virulence. Altogether， these findings indicated that the global regulators FNR and Fur and the signaling transduction system Rcs coordinately regulated the expression of ECP biosynthesis genes leading to increased ExPEC serum resistance in response to low oxygen and low iron levels in the bloodstream. This article is protected by copyright. All rights reserved.
