| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The lysed and neutralized sample was added with 5 μl of a 400 μM solution of random primers (Genscript, Nanjing, China), 6 μl of 10× PCR buffer (Takara, Dalian, China), 3 μl of a mixture of the 4 dNTPs (each at 2.5 mM), and 1 μl of Taq polymerase (Takara, Dalian, China) and brought to 60 μl with water. | Get A Quote |
A recently developed adenine base editor (ABE) efficiently converts A to G and is potentially useful for clinical applications. However， its precision and efficiency in vivo remains to be addressed. Here we achieve A-to-G conversion in vivo at frequencies up to 100% by microinjection of ABE mRNA together with sgRNAs. We then generate mouse models harboring clinically relevant mutations at Ar and Hoxd13， which recapitulates respective clinical defects. Furthermore， we achieve both C-to-T and A-to-G base editing by using a combination of ABE and SaBE3， thus creating mouse model harboring multiple mutations. We also demonstrate the specificity of ABE by deep sequencing and whole-genome sequencing (WGS). Ta... More
