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Evaluation of the AID carbapenemase line probe assay for rapid detection and identification of carbapenemase genes in Gram-negative bacilli.

J. Antimicrob. Chemother.. 2017; 
BloembergGuido V,Braun-KiewnickAndrea,TedrupJan,MeijerinkCarla,DurerElena,RitterClaudia,KellerPeter M,HombachMic
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PCR Cloning and Subcloning Genes for blaBIC (885bp; GenBank accession number GQ260093.1), blaSIM (741bp; GenBank accession number GQ288397.1), blaDIM (756bp; GenBank accession number KC004136.2), blaIMI-1 (879bp; GenBank accession number JX090311.1), blaIMI-3 (879bp; GenBank accession number GU015024.1) and blaNMC-A (879bp;GenBankaccessionnumberZ21956.1)weresynthesizedandintroduced into the multiple cloning site of plasmid pUC57-kan (GenScript, Piscataway, NJ, USA). Get A Quote

摘要

This study evaluated the AID carbapenemase line probe assay (LPA) for the detection and identification of carbapenem resistance genes in Enterobacteriaceae and other Gram-negative bacilli (GNB) using bacterial cultures and DNA extracts directly from patient urine samples.

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