| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | TheHA1geneofA/Shanghai/2/2013was synthesized(Genscript,Nanjing,China),fusedwiththeFcfragmentofhumanIgG1andaC-terminalAvi-tag(GLNDIFEAQKIEWHE),and cloned into pSecTag2B expression vector (Thermo Fisher). The recombinant plasmid HA1-Fc-Avi was transfected into Expi293 cells for transient expression and used for biopanning. The baculovirus-expressed HA1 was produced by cloning synthesized A/Shanghai/2/2013 H7N9 HA1 gene adjacent to a C-terminal 6-His tag into pAcGP67a vector and expressed in Sf9 insect cells according to the manufacturer’s instructions. The trimeric HA was generated by fusing a T4 phage fibritin-derived trimeric foldon (GYIPEAPRDGQAYVRKDGEWVLLSTFL) to the C-terminal of synthesized A/Shanghai/2/2013 H7N9 HA gene (Genscript), and transiently expressed using Expi293 Expression System | Get A Quote |
The H7N9 influenza virus causes high-mortality disease in humans but no effective therapeutics are available. Here we report a human monoclonal antibody， m826， that binds to H7 hemagglutinin (HA) and protects against H7N9 infection. m826 binds to H7N9 HA with subnanomolar affinity at acidic pH and 10-fold lower affinity at neutral pH. The high-resolution (1.9 Å) crystal structure of m826 complexed with H7N9 HA indicates that m826 binds an epitope that may be fully exposed upon pH-induced conformational changes in HA. m826 fully protects mice against lethal challenge with H7N9 virus through mechanisms likely involving antibody-dependent cell-mediated cytotoxicity. Interestingly， immunogenetic analysis in... More
