| Products/Services Used | Details | Operation |
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| PCR Cloning and Subcloning | Infected cell lysates or purified proteins were separated on 15% acrylamide/bisacrylamide gels (BioRad mini-protean tetra cell apparatus) and transferred to nitrocellulose membranes for 120min using a mini-protean transblot cell and Tris glycine transfer buffer. Following blocking with 5% non-fat milk, primary antibody incubations were carried out overnight at 4C using 1:1,000 diluted anti-2A (rabbit polyclonal raised against the C-terminal 14 aa of 2A by GenScript) or anti-tubulin (rat monoclonal; Abcam, ab6160) antibodies. | Get A Quote |
Programmed -1 ribosomal frameshifting is a mechanism of gene expression， whereby specific signals within messenger RNAs direct a proportion of translating ribosomes to shift -1 nt and continue translating in the new reading frame. Such frameshifting normally occurs at a set ratio and is utilized in the expression of many viral genes and a number of cellular genes. An open question is whether proteins might function as trans-acting switches to turn frameshifting on or off in response to cellular conditions. Here we show that frameshifting in a model RNA virus， encephalomyocarditis virus， is trans-activated by viral protein 2A. As a result， the frameshifting efficiency increases from 0 to 70% (one of th... More
