Aestivation Induces Changes in the mRNA Expression Levels and Protein Abundance of Two Isoforms of Urea Transporters in the Gills of the African Lungfish, Protopterus annectens.
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Two rabbit polyclonal antibodies against aa 23– 36 (HNTEKQNDKKESVKC) of Ut-a2a and aa 40–53 (LSENTETSVPEYHPC) of Ut-a2b from P. annectens were custom-made through a commercial company (GenScript, Piscataway, NJ, USA). The concentration of the anti-Uta2a and anti-Ut-a2b antibodies used for Western blotting was 1.7µgml−1. |
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ammonia, nitrogenous waste, urea excretion, Ut-a2a, Ut-a2b