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Aestivation Induces Changes in the mRNA Expression Levels and Protein Abundance of Two Isoforms of Urea Transporters in the Gills of the African Lungfish, Protopterus annectens.

Frontiers in Physiology. 2017-02; 
You R. Chng, Jasmine L. Y. Ong, Biyun Ching, Xiu L. Chen, Kum C. Hiong, Wai P. Wong, Shit F. Chew, Siew H. Lamand Yuen K. Ip
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PCR Cloning and Subcloning Two rabbit polyclonal antibodies against aa 23– 36 (HNTEKQNDKKESVKC) of Ut-a2a and aa 40–53 (LSENTETSVPEYHPC) of Ut-a2b from P. annectens were custom-made through a commercial company (GenScript, Piscataway, NJ, USA). The concentration of the anti-Uta2a and anti-Ut-a2b antibodies used for Western blotting was 1.7µgml−1. Get A Quote

摘要

关键词

ammonia, nitrogenous waste, urea excretion, Ut-a2a, Ut-a2b