Newcastle disease virus-based H5 influenza vaccine protects chickens from lethal challenge with a highly pathogenic H5N2 avian influenza virus
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PCR Cloning and Subcloning> |
Standard hematoxylin and eosin (H&E) slide staining for the collected tissues was performed and influenza nucleoprotein (NP) was detected in the tissues by immunohistochemistry (IHC) assay using a Rabbit Anti-H1N1 NP polyclonal antibody (GenScript, NJ) as described in our prior study.18 H&E stained tissues and immunohistochemistry were reviewed by a veterinary pathologist in a blinded fashion. |
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