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Mutagenesis Services> | The VAMP3(D71)-mCitrine mutant was generated by site directed mutagenesis. For FKBP-Stx4-mCitrine, synthetic DNA coding for human FKBP1A residues 1–108 with a serine-glycine linker (SGGGGSGGGGSGGGG) (Genscript, Piscataway, NJ) was subcloned in the restriction sites XhoI/EcoRI of Stx4-mCitrin | Get A Quote |
Cluster of differentiation 24 (CD24) is a specific surface marker involved in the tumorigenesis and progression of hepatocellular carcinoma (HCC). However, all reported anti-CD24 antibodies are murine ones with inevitable immunogenicity. To address this, a method using both molecular structure and docking-based complementarity determining region (CDR) grafting was employed for humanization. After xenogeneic CDR grafting into a human antibody, three types of canonical residues (in the VL/VH interface core, in the loop foundation, and interaction with loop residues) that support loop conformation and residues involved in the antigen-binding interface were back-mutated. Four engineered antibodies were produced, am... More