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Expression optimization of a cell membrane-penetrating human papillomavirus type 16 therapeutic vaccine candidate in Nicotiana benthamiana

Scientific Reports,. 2017; 
Renata de A. B. Assis Lorraine Cristina Polloni, José S. L. Patané, Shalabh Thakur, Érica B. Felestrino, Julio Diaz-Caballero, Luciano Antonio Digiampietri , Luiz Ricardo Goulart, Nalvo F. Almeida , Rafael Nascimento, Abhaya M. Dandekar, Paulo A. Zaini João C. Setubal, David S. Guttman & Leandro Marcio Moreira
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Mutagenesis Services Te mutagenesis cassette was chemically synthesized (GenScript, USA) afer insertion of a kanamycin resistance gene in the central portion of the target gene XAC0501 (within Ser-200, the frst amino acid of the active triad, maintaining the 5′ and 3′ unchanged to allow homologous recombination). Get A Quote

摘要

The Xanthomonadaceae family consists of species of non-pathogenic and pathogenic γ-proteobacteria that infect diferent hosts, including humans and plants. In this study, we performed a comparative analysis using 69 fully sequenced genomes belonging to this family, with a focus on identifying proteins enriched in phytopathogens that could explain the lifestyle and the ability to infect plants. Using a computational approach, we identifed seven phytopathogen-enriched protein families putatively secreted by type II secretory system: PheA (CM-sec), LipA/LesA, VirK, and four families involved in N-glycan degradation, NixE, NixF, NixL, and FucA1. In silico and phylogenetic analyses of these protein families revealed... More

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