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Activation mechanism and cellular localization of membrane-anchored alginate polymerase in Pseudomonas aeruginosa

Accecpted Manuscript Posted Online. 2017; 
M. Fata Moradali, Shirin Ghods, Bernd H. A. Rehm *
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Mutagenesis Services Relevant encoding genes (i.e., alg8, alg8-12His, alg44, and alg44-12His) for heterologous production were optimized based on E. coli codon usage and synthesized by GenScript Get A Quote

摘要

The exopolysaccharide, alginate, produced by the opportunistic human pathogen 28 Pseudomonas aeruginosa represents a survival advantage by contributing to formation of 29 characteristic biofilms during infection. Membrane anchored proteins Alg8 (catalytic subunit) 30 and Alg44 (co-polymerase) constitute the alginate polymerase which is being activated by the 31 second messenger molecule c-di-GMP, but the mechanism of activation remains elusive. To 32 shed light on the c-di-GMP mediated activation of alginate polymerization in vivo, an in 33 silico structural model of Alg8 fused to the c-di-GMP binding PilZ domain informed by the 34 structure of cellulose synthase, BcsA, was developed. This structural model was ... More

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