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Degradation of lignin β‐aryl ether units in Arabidopsis thaliana expressing LigD, LigF and LigG from Sphingomonas paucimobilis SYK‐6

Plant Biotechnology Journal. 2016; 
Ewelina Mnich , Ruben Vanholme, Paula Oyarce Sarah Liu , Fachuang Lu , Geert Goeminne, Bodil Jørgensen , Mohammed Saddik Motawie , Wout Boerjan, John Ralph , Peter Ulvskov , Birger Lindberg Møller, Nanna Bjarnholt and Jesper Harholt
Products/Services Used Details Operation
PCR Cloning and Subcloning For expression of the various Lig genes in Escherichia coli and Arabidopsis thaliana, genes were synthesized by GenScript (Hong Kong). For expression in E. coli the following genes were synthesized: LigD (DDBJ accession no. D11473–1), LigF (DDBJ accession no. D11473-2) and LigG (DDBJ accession no. AB026292) and sub-cloned into the pETSTREP vector (Dixon et al., 2009). Get A Quote

摘要

Lignin modification in planta by Bacterial Enzymes

关键词

Biofuel, lignin modification, bacteria, Sphingomonas paucimobilis, Lig β-aryl ether, Saccharification yield
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