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Rational Modular RNA Engineering Based on In Vivo Profiling of Structural Accessibility

ACS Synth. Biol. 2017; 
Lydia M. Contreras
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Mutagenesis Services The 21- member rational library of CsrB variants was built by site directed mutagenesis, single-fragment Gibson Assembly, or purchased from Genscript. Construction method, primers, and CsrB sequence for each pCML 877-derived plasmid are documented in Table S1 Get A Quote

摘要

Abigail N. Leistra, Paul Amador, Aishwarya Buvanendiran, Alex Moon-Walker,and Lydia M. Contreras

关键词

rational RNA engineering, RNA design, modular recombination, engineering gene regulation, regulatory network, post-transcriptional control
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