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Peptide Synthesis> | … 02115, USA *To whom correspondence should be addressed. E-mail: joseph_loparo@hms. harvard.edu and … Materials and Methods Chemicals Custom synthesized biopolymers were obtained from Genscript or IDT, and are listed in Table S2 in the Supporting Information … | Get A Quote |
Bacterial Expression System> | Get A Quote |
Regulated proteolysis of signaling proteins under mechanical tension enables cells to communicate with their environment in a variety of developmental and physiologic contexts. The role of force in inducing proteolytic sensitivity has been explored using magnetic tweezers at the single-molecule level with bead-tethered assays, but such efforts have been limited by challenges in ensuring that beads not be restrained by multiple tethers. Here, we describe a multiplexed assay for single-molecule proteolysis that overcomes the multiple-tether problem using a flow-extension strategy on a microscope equipped with magnetic tweezers. Particle tracking and computational sorting of flow-induced displacements allow as... More