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Production of santalenes and bergamotene in Nicotiana tabacum plants.

PLoS ONE. 2019; 
YinJun-Lin,WongWoon-
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PCR Cloning and Subcloning … RTPFPS, FPS, and SaCYPF39v1 (GenBank: KC533716.1, synthesized by Genscript) were cloned into pBA002 vectors to generate pBA … cloned into pB7WG2D vectors to generate pB7WG2D-tHMGR and pB7WG2D-HMGR, respectively, via Gateway cloning (Invitrogen, USA) … Get A Quote

摘要

Terpenes play an important role in plant-insect relationships, and these relationships can potentially be modified by altering the profile of terpenes emitted from plants using metabolic engineering methods. Transgenic plants generated by employing such methods offer the prospect of low-cost sustainable pest management; in this regard, we used chloroplast targeting and cytosolic mevalonic acid pathway enhancement in this study to investigate the interaction of santalenes and bergamotene with insects. The santalene- and bergamotene-emitting transgenic tobacco plants thus generated were utilized to study host preference in the green peach aphid (Myzus persicae (Sulzer)). The results showed that co-expression ... More

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